Unfortunately, the snake gobbles up all the frogs and Anne asks Sasha what to do, but Sasha nervously insists that Anne should stay in charge and they do whatever she thinks is best.Īt this point, Anne demands Sasha take over again, but Sasha finally reveals her insecurity over doing so and when Anne asks why she won't lead them, Sasha explains that she ruined their friendship the last time she was in charge. When the noise of their argument attracts the attention of a mind-controlled snake, Anne orders the frogs to attack, they ask for a specific kind of attack and Anne uncertainly tells them to do what feels right. Sasha denies there being any angle, but struggles to give a straight answer. Sasha manages to save the frogs and get them to the other side, but by this point Anne officially confirms her suspicions that she's not a good commander, and finally breaks asking Sasha what her angle is. They fell into the trench because Anne didn't measure the trench width, something Sasha asked her about. A montage shows that the mission goes poorly under Anne's command, from nearly tasting river water polluted with waste to walking into quick sand after being distracted by watching a show on Anne's phone where they escape a frobot ambush, and looking through the wrong end of a telescope, leading them into another frobot ambush.Īfter finally arriving at the factory, separated from them by a trench, Anne's idea is for the troops to make a bridge of themselves to cross it after failing to notice a useful grappling hook in Sasha's possession. By this point, Anne is even more uncertain she is fit to lead and asks if Sasha should take charge again, which the latter denies. Before the mission even starts, Anne has forgotten a map and is about to rush back to base to get it, which Sasha already got with all the major points labeled. The new team sets out on their first mission to destroy a certain factory to stop the manufacturing of the mind control collars that are found on the wildlife. After Sasha briefs Anne and the Plantars about an upcoming mission that takes place the next morning, Anne leaves and Grime enters, shocked that Sasha gave Anne the helmet asking why she hand over control to to Anne since she has no experience leading an army but Sasha is being stubborn and vague about her decision. Anne is quite uncertain about this due to how much has changed since the last time she was in Amphibia, but Sasha rallies support from the rest of the resistance to anoint Anne as the new leader. We are then introduced to the helmet of authority that Sasha is wearing, who then bequeaths it upon Anne since the hopes that she would return is what kept the town going this whole time. Anne accepts this and agrees to her and Sasha being on the same side for a change and promises they will deal with their emotional baggage when it's more convenient. Hop Pop, however, is having a hard time adjusting to his old life back home after being spoiled by the comforts of Earth.Īnne and Sasha briefly talk about the events at Newtopia Castle, where Sasha talks about how she has changed and her days of toxic manipulation are over. The town's residents all moved into the tunnels as well and became The Resistance, all of whom rejoiced when Anne and the Plantars returned. Sasha informs them of Marcy being alive and kept prisoner by Andrias, whose army has taken over Amphibia and destroyed towns including Wartwood. The Plantars' home serves as the entrance to the ancient family tunnels that have been turned into the rebel's secret hideout. As Anne and the Plantars are threatened by one of these creatures, mysterious figures come out and save them as well as the creature, removing the mind control device on its neck and reveal themselves to be some of the familiar residents of Wartwood, led by Sasha. PlotĪnne is shocked from seeing the new Loggle.Īnne and the Plantars arrive back in Amphibia only to find that Wartwood has been attacked and nearly destroyed by King Andrias and his army, as well as some wildlife under mind control. Anne is made leader of the Wartwood Resistance, even though she's not good at the job.
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Hopefully, your sketching will remain a joyful experience for hours on your iPad. As a result, your fingers won’t feel tired even after a long session of note-taking or drawing. The cover has an ergonomic design, offering the desired comfy hold. So, you can easily find a stunning match for your digital pen. And you have a ton of captivating colors to pick from. Talking about the features that make it a worthy suit for your digital pencil is the modish design. It has received four and a half stars out of 185 customer reviews (at the time of writing this article). JIAJUN is one of the most popular Apple Pencil cases on Amazon. Our Main Inn Rooms, Second-Floor Balcony Rooms, Guest Rooms on Cottage Hill, and Veranda Rooms h ave everything you need to relax and unwind. Loose Shoe Brewing Company | Amherst, VA | 50 minutesĪfter you’ve (responsibly) tasted your way through Virginia’s Blue Ridge, return to your home base at Natural Bridge Hotel.Beale’s Brewery | Bedford, VA | 48 minutes.Rockbridge Vin e yard & Brewery | Raphine, VA | 30 minute s.Heliotrope Brewing | Lexington, VA | 22 minutes.If you’re willing to drive a little further for some unique flavors, check out these breweries less than an hour away from Natural Bridge : The hillside views don’t hurt, either!ĭrive time: 20 minutes | Directions More Options Nearby Check out the Outpost Taproom & Kitchen where you can indulge in handcrafted brews and delicious food. This state-of-the-art brewery is known for its award-winning lagers brewed right here in the heartland of Virginia. Next up is Devils Backbone Brewery, a local haunt that was recently acquired by Anheuser-Busch. There’s something for everyone!ĭrive time: 10 minutes | Directions 3. Tip: If there’s a wine lover in your group, they’ll be happy to know that Great Valley also produces Cabernet Franc, Gruner Veltliner, Lemberger, and Vidal Blanc varietals. Choose your brew from the tasting room, then head outside to the patio where food trucks and live music await. Here, you’ll find top-quality Belgian ales, IPAs, German lagers, and more. Great Valley Farm is just a mile down the road from Ha lcyon and should be the next stop on your list. Be sure to stop by the pavilion for incredible 360-degree views of the Blue Ridge Mountains. You can s ip your cider while walking through the apple orchard and take in the s ights and smells. Their vibrant, naturally gluten-free ciders are made from 100% Virginia – grown apples and have no artificial flavors, sweeteners, or colors. The first and closest brewery to us isn’t quite a brewery – it’s a cidery! Halcyon Days is a great place to start your beverage tour. Get Your Free Guide 7 of th e Top Breweries Near Natural Bridge, VA 1. Our guide has local recommendations for restaurants, shops, outdoor recreation, and more. Then, use our exclusive Vacation Guide to map out the rest of your trip. Ĭheck out some of our favorit e Natural Bridge breweries below. And, when you stay at Natural Bridge Hotel, you’ll be just minutes away from the best local hangouts. Virginia’s Blue Ridge offers tasty brews served with spectacular mountain views. Beer lovers, unite! Whether you a re a craft beer aficionado looking for the next best thing or want to enjoy a casual cold one, you’ll love the breweries near Natural Bridge, VA. This means you can be lining up dark energy lances and summoning shields while you slash. I seem to be constantly finding new moves and thinking tactically about how to unravel enemies with my skill combinations.Īs well as swords and spears, the protagonist can leverage his talking book sidekick Grimoire Weiss to use dark magic to deal with foes, and these attacks are mapped to the bumpers. It’s frantic due to the bullet hell projectiles that spew from the game’s enemies, but it doesn’t feel mindless. Luckily, it has been revised in this remake to be closer to its sequel, and now it doesn’t feel at all out of date. The original NieR has story in spades, but the combat paled in comparison. NieR Automata’s combat was handled by PlatinumGames of Bayonetta fame, which meant it had show-stopping gameplay and an equally compelling narrative. The first main point of contention for NieR fans who may have wanted to play the original after Automata was the controls. This 2021 remake, or “version upgrade” as director Yoko Taro calls it, wants to delight veterans and introduce new fans like me to the game that laid the groundwork for Automata. You see, NieR originally launched on PS3 and Xbox 360 all the way back in 2010 and gained quite the cult following. Like many fans, I came to the series with 2017’s NieR Automata, and have been endeavouring to work my way backwards ever since. NieR Replicant ver.1.22474487139… Credit: Square Enix What followed was a truly mind-blowing bit of storytelling that shouldn’t be spoiled here, but I hope that gives you an inkling of what makes NieR so special. I knew that my character’s mood had darkened and that his partner Grimoire Weiss was startled, but I, the player, had lost control. The mayor and the protagonist’s actions and dialogue then started to be laid out on screen as text, as if I was reading a play. I could feel an unsettling pit in my stomach as my brain tried to parse what was happening. Slowly, my screen started to fade out until there was nothing but black. He warned me about a contagion of words, and as I spoke to him about it, the mood shifted dramatically. Here I met the mayor of this place, who seemed distant, caught in a trance. READ MORE: ‘NieR’ composer Keiichi Okabe: “I always conveyed the tragic fates that the protagonist and characters are burdened with”Īn expressionless shade of the game’s protagonist mouthed words that drew me to a misty forest, just one peculiar pocket of the game’s endlessly interesting world.After spending hours engaging with the game’s unique take on RPG gameplay – roaming the melancholy plains, meeting endearing characters and fighting monsters that drop schoolbooks and broken watches – I was met with a terrible dream. N ieR Replicant is full of moments that are bursting with intrigue, but one that has really stuck with me was my first trip to the Forest of Myth. Medical News Today have selected the top 10 apps to assist with stroke recovery. They can help you or your loved one to track appointments and medications, provide language therapy, train the brain, and even lower some risk factors for future strokes. Apps are available to help aid the stroke recovery process. While some people recover quickly from stroke, others may need long-term support. Surveys have shown that people who “arrived at the emergency room within 3 hours” of their first symptoms of stroke had “less disability” 3 months later than those who were treated later. It’s also available at the New York Times, USAToday, The Washington Post, and The Los Angeles Times. It’s available through a number of different sites, including both Web Sudoku and (which both have iOS and Android apps available). The faster a person is treated after stroke, the more likely they are to recover from it. Sudoku is one of the most popular brain training games online. Stroke can also trigger mental health issues such as anxiety and depression, as well as movement and coordination problems, paralysis, difficulties swallowing, visual impairment, and urinary incontinence and loss of bowel control. Stroke can cause significant injury to the brain that may result in many long-term problems.įor example, communication, concentration, memory, and executive function, as well as spatial awareness, are all cognitive functions that may be impacted by stroke. If you are not sure how to use it, there will be pre-set training programs for 8 different sports (baseball, basketball, soccer, football, lacrosse, tennis, hockey, and volleyball) that will constantly be updated, or you can check out our social media pages for ideas. This app also provides a feedback system that includes the ability to measure and track response time and accuracy, as well as post-training self-evaluation questions that are designed to measure and track your effort levels and increase self-awareness about your performance.Ischemic strokes - wherein “blood flow to the brain is blocked” - account for roughly 87 percent of all strokes. Currently being used by top pro teams around the world, SwitchedOn® provides PCM training with the convenience of your smartphone that can be done essentially anywhere, with little to no other equipment needed. This is called Perceptual Cognitive Motor (PCM) training, and is designed to improve physical and cognitive performance simultaneously in a way that transfers to competition. Users associate sports-specific actions or movements with the randomized cues that the app provides (colors, numbers, or arrows), which forces them to PERCEIVE the external stimulus, process that information to DECIDE what action it was associated with, and then to REACT as quickly and efficiently as possible, which is similar to what they have to do during games. The app aims to improve memory, attention, problem-solving, and mental math skills. It allows them to practice their physical and technical skills in a reactive game training environment that replicates what is experienced during competition. Impulse is a free brain-training app, although users can make in-app purchases to remove the ads. SwitchedOn® is essential for any athlete looking to take their game to the next level. These cognitive skills are what separate the good from the great, and must be trained in conjunction with your body in order to have best transfer to competition. You must have the cognitive skill to quickly process external information and make split-second decisions in order to use your physical skills successfully during games. Individual training involves performing pre-determined actions where you know what you will do and when you will do it, but playing in games requires the exact opposite. This is especially true with individual training which all of the greatest athletes in the world will tell you is essential to get ahead of the competition. Performance analysis and feedback to record and track progress WHY USE SWITCHEDON?ĥ0 to 90 percent of sports is mental, yet the training done by most athletes does not reflect this. Improved speed, agility, reactions, and athletic performanceĮasily applicable for individual, partner, or team training Better transfer from individual training to competitionĮnhanced focus, awareness, decision making, cognitive processing, and memory Simply being at this stage is already a no-win scenario for most merchants. For merchants, this would mean an opportunity to accept the chargeback before further fees and final judgments apply. Remember, the pre-arbitration (or dispute response) stage is a “last chance” to resolve the issue before moving on to the arbitration stage. Certain conditions apply, and Visa only allows a 30-day timeframe (individual acquirers typically set even tighter deadlines).īroken down, it would look something like this:Ĭan Merchants Fight Back Against Arbitration Cases?Īt this stage, your only option is to either proceed to card network arbitration, or accept the losses from the initial chargeback. If Visa rules in favor of the cardholder, acquirers and merchants have a limited ability to challenge. Pre-Arbitration Chargebacks in the Allocation Workflow There’s an “Allocation” track for fraud and authorization issues, and a “Collaboration” track for consumer and processing errors. Since implementing Visa Claims Resolution rules, all Visa disputes are divided into one of two workflows. Most obviously, they refer to this step as the pre-arbitration stage, or dispute response, rather than the arbitration chargeback stage. Visa does things a little differently from Mastercard. They have the right to do this until the case decision is finalized. The issuer, by the way, can respond to any new information you provide at any time. So, if you have 45 days to respond to an arbitration case, you can count on having 20-22 days to get documentation and evidence to the acquirer. They do this because they need time to review everything and get it to Mastercard before the deadline. This is somewhat misleading since your acquiring bank will probably have a more stringent timeline.Įach acquirer is different, but your response time is typically 50-65% of the card scheme's limit. In the case of an arbitration chargeback, though, the issuer must file within 45 calendar days of the second presentment settlement date. A few reason codes allow for shorter timeframes. For example, most cardholders can only file a chargeback within 120 calendar days of the initial transaction. Specific time limits are associated with every aspect of the chargeback process. Section A | Mastercard Arbitration Time Limits Your best bet is to carefully review Mastercard’s policies and regularly check for updated regulations, so you're always aware of the latest requirements. You never really know when a second presentment will turn into an arbitration case filing. Mastercard’s chargeback process is constantly evolving. We'll start with Mastercard's process, which can be divided into four main stages leading up to arbitration: Mastercard Chargeback Arbitration Processīefore we focus on arbitration, let's review the steps that lead up to it. With that in mind, here are some things you should expect from the arbitration chargeback process, based on the card brand. The specific steps, time frames, and terminology are different, though. Mastercard and Visa have similar regulations regarding the chargeback arbitration process. If you have strong evidence to support your case, though, it could be well-worth the trouble. To them, it’s better just to take the chargeback than risk losing even more. Many merchants choose not to go into arbitration because of the added fees, time, and potential penalties if you lose your arbitration case. You have two options at this point: either accept the arbitration case, or appeal to an arbitrator from the card network to take another look. For a Visa transaction, this is called a pre-arbitration. If the evidence isn’t strong enough, the cardholder’s issuing bank might reject your case. The cardholder makes an allegation (the chargeback), then you provide evidence in response to counter that allegation. The chargeback arbitration process works just like litigation in the legal system. Disputes that require arbitration will rarely go well for you as a merchant, though. Most disputes get settled before they reach the arbitration phase. So, a representative of the card scheme is asked to intervene and make a judgment. The difference is that, in the payments industry, it's the card network who makes a decision, rather than a jury or a judge.īy this stage, the parties involved-the bank, cardholder, or the merchant-cannot resolve a dispute on their own. Visa uses the term "pre-arbitration" for this response.Īs with a legal case, arbitration happens when two parties can't resolve a dispute and need an independent arbiter. In this case, the cardholder submits further evidence to convince Mastercard to intercede and resolve the chargeback. This is filed after a merchant already won their representment and was granted a return from the original transaction. A chargeback arbitration case filing is Mastercard’s term for a response to a chargeback. This condition may cause weight changes or swelling and tightness around the neck.Īnyone experiencing these symptoms should stop consuming iodine and consult their doctor for a full evaluation. While many people can handle high levels of iodine, some are more vulnerable to its effects, which can include hyperthyroidism. Most seaweed contains high levels of iodine, and a person may consume too much if they eat a lot of seaweed over an extended period. There are several factors a person should be aware of when adding seaweed to their diet, including: Excess iodine Plus, the high antioxidant content of many types of algae may support heart health over time. The body then uses cholesterol to replace these elements, which may decrease a person’s total cholesterol by up to 18%. These soluble fibers bind to bile acids or salts in the body. It may protect the heartĪs the same study notes, high-fiber foods such as algae may also reduce cholesterol levels. As a result, the stomach may delay sending signals of hunger to the brain, which may help prevent overeating. This is because fiber is low in calories and helps a person feel full.Īccording to the study in Marine Drugs, a high amount of dietary fiber delays stomach emptying. The fiber in seaweed may benefit individuals who are trying to lose weight. Intestinal bacteria break fiber into compounds that improve gut health and the health of the immune system.Īdding algae to the diet may be a simple way to provide plenty of gut-healthy prebiotic fiber, which can help people experiencing issues such as constipation or diarrhea. This fiber can help feed the gut’s bacteria. Authors of a study in the Journal of Applied Phycology report that algae tend to contain high amounts of fiber, which may make up 23–64% of the algae’s dry weight. It may support gut healthīacteria in the intestines play an important role in breaking down food and supporting digestion and overall health.Īlgae may be an ideal food for the gut. Further research in humans may help provide stronger evidence for using these compounds. Adding seaweed to the diet may help boost a person’s fiber intake without a large increase in calories.Ī 2018 study in rats found that compounds in one type of seaweed may directly reduce markers of type 2 diabetes, such as high blood sugar.Ĭompounds in seaweed may also reduce diabetes risk factors, such as inflammation, high fat levels, and insulin sensitivity. This is because high amounts of fiber help regulate blood sugar levels and insulin levels. It may help with diabetesįiber-rich foods may help with diabetes. This occurs when an overactive thyroid gland produces excessive amounts of hormones. However, excessive iodine intake may worsen a person’s symptoms of hyperthyroidism. It can also cause a goiter, which is a visible enlargement of the thyroid gland. Iodine deficiency is one cause of hypothyroidism, an underactive thyroid that can cause symptoms such as fatigue, cold intolerance, and sore muscles and joints. Some seaweed dietary supplements may not provide adequate iodine, while others offer more than the Daily Value. The type of seaweed and the location in which it was grown can alter the iodine contents, which can make it an unreliable source of iodine.Īccording to research from 2021, the consumption of several whole food seaweed or food products containing seaweed can cause excess iodine intake. The thyroid needs iodine to function correctly, but the amount that a person requires depends on the health of the thyroid. The thyroid gland controls and releases hormones for energy production, growth, and cellular repair. Seaweed also contains antioxidants, which may protect the body from oxidative stress and reduce inflammation at the cellular level. It is highly nutritiousĮach type of seaweed may contain slightly different nutrients and minerals.Įating marine alga may be a simple way to boost a person’s intake of vitamins and minerals without adding many calories.Īs a study in Marine Drugs notes, seaweed is generally a good supply of:Ī study in the Journal of Applied Phycology notes that various types of seaweed contain helpful nutrients, including: The following are the best health benefits of seaweed: 1. Share on Pinterest Paul Taylor/Getty Images The program now supports Mac OS 10.9 and above and Windows 7 and later. Movavi Screen Recorder offers the ability to easily set up recording parameters, grab audio and video, and edit the recording and save the file. New Screen Recorder 11: completely redesigned, enhanced and answers all your. Does the screen recorder you’re interested in have a system of support? Can you submit a ticket if something goes wrong, and is software regularly updated to keep up with OS updates? A lot of the freeware options or open-source recorders can come up short when it comes to support, so they may not be best for making professional recordings. OS: Windows 7/8/10/11, Mac OS 10.13 or higher. If there are editing features, consider how simple these are to use. Some software will even let you use key commands and shortcuts to start making recordings quickly and easily. Is software easy to install and start using? You want something that is convenient and can allow you to make recordings in a matter of minutes, rather than taking ages to set up. Movavi Screen Recorder has been designed by professionals who understand what’s important in a screen capture app. For instance, if you want to show this video again at a future date and broadcast it on a big screen, like a TV screen, then you should definitely try to record in the highest quality you can.Įase of use. Movavi Screen Recorder is a handy app that enables you to record your screen and grab screenshots right from your computer screen. How good does the quality need to be? Both audio and video quality should be considered. Do you need to edit your video or not? Some screen recorders let you cut the beginning and end off your video, while some allow more advanced editing. Inicie el programa y configure los parámetros de grabación. However, if you want to add notes or keystrokes, think about a screen recorder with an annotation feature.Įditing options. Consulte la siguiente guía breve sobre cómo realizar una captura de pantalla en Mac. If not, you may be satisfied with one of the browser-based recorders or even browser add-ons and plug-ins.ĭo you need to annotate? A lot of the time you will just be recording a video for future reference without the need of any form of annotation or explanation. If you do, you can install a desktop program, like Movavi Screen Recorder, that provides you with a lot of features and functions to ensure that you have a polished end-product. A Skype call can be made on both your mobile devices and computer once you’ve downloaded the Skype app. Fortunately, there are lots of options and methods to choose from:ĭecide whether you want to install software. Use this step-by-step guide to show you how to record a Skype video call in a few clicks. For example, if you just want to make a quick five-minute explainer video, you may not be worried about features and functions, such as editing or annotation. Download Movavi Screen Recorder for Windows to capture and record video from the screen. Download Movavi Screen Recorder using the correct installation version for either a PC or Mac, and then follow the instructions to install and launch the software. Choosing a screen recorder should be based on your own specific needs. She may have fallen short in her bid to become the grand winner of that season, but that didn’t hinder her from pursuing her musical career. She won the daily rounds and was able to clinch the golden microphone on that day, and went on to advance to the semifinals after being the defending champion for five consecutive days. A Luzon contender then from Calamba, Laguna, she immediately captured the hearts of everyone not just with her pretty and angelic face, but with her mellifluous rendition of the Nonoy Zuniga’s “ Araw Gabi”, which was also covered by Asia’s Songbird Regine Velasquez-Alcasid in 2004. She then went on to perform in various activities and join different contests growing up, as well as served as a vocalist in a band called Serenata’s New Perspective.Īnd when TNT was relaunched five years ago, she didn’t hesitate to give it a shot not for fame nor fortune, but to simply satisfy her desire to perform onstage. Unlike other singers who have gone through rigorous formal training to develop their talents, she revealed that her vocal prowess just came out naturally. However, she’s actually been singing since she was five years old, as she disclosed in a media interview in that same year. Since she’s probably new to others, get to know her a bit more in this feature!īorn Mary Gidget Dela Llana, her fans know that her journey in local entertainment started when she joined the inaugural season of It’s Showtime’s Tawag Ng Tanghalan in 2016. And one of them is RISE Artists Studio talent and Tawag ng Tanghalan alumna Gigi De Lana. Last June 19, avid supporters of ABS-CBN and its artists witnessed the historic Star Magic Black Pen Day, wherein more than 40 celebrities inked their deals with the network’s aforementioned talent management arm. Amino acids in proteins are rarely found in backbone conformations with positive φ and ψ angles at the α L region of Ramachandran space. Īnother practical application is the development of thermostable proteins that incorporate D-amino acids. Methods are being developed for incorporating L and D amino acids in computational de novo protein design. Other microbial peptides such as tolaasin use D-amino acids to enforce sharp bends in an α-helical domain. The β-helix has been used as the foundation for novel cyclic peptide folds and peptide nanotubes with ion channel activity and antimicrobial properties. This allows it to adopt the β-helix, a novel secondary structure composed of alternating positions in the β L and β R conformation. The antimicrobial toxin, gramicidin, is a well studied example of such a molecule, containing alternating L and D amino acids. This work has been motivated by natural examples of polypeptides that combine L and D amino acids. Much of the work on the role of variable stereochemistry on structure and stability has been conducted on short peptides. In this study, we use the Protein Data Base (PDB) as a source of structural information for specific D-amino acid sidechain interactions with α-helical backbones. The resulting enantiomeric molecules were both well-folded and specifically active on a protease substrate of the same respective amino acid chirality as the enzyme. A dramatic example was the chemical synthesis of the ninety-nine amino acid long HIV-1 protease from both L and D-amino acids. This has allowed protein chemists to explore the physical and biological effects of varying amino acid stereochemistry. The field has developed rapidly, permitting the construction of synthetic, protein-sized molecules. Solid phase chemical synthesis allows for the incorporation of non-natural amino acids into polypeptides. These will provide a basis for de novo design of novel heterochiral protein folds. Conclusionīy examining left-handed α-turns containing L-amino acids, new interaction motifs for incorporating D-amino acids into right-handed α-helices are identified. Helix capping sidechain-backbone interactions are found which are unique to α L helices including an elevated propensity for L-Asn, and L-Thr at the amino terminus and L-Gln, L-Thr and L-Ser at the carboxy terminus. Two backbone rules for terminating a left-handed helix are found: an α R conformation is disfavored at the amino terminus, and a β R conformation is disfavored at the carboxy terminus. Propensities for amino acids to occur in contiguous α L helices correlate with published thermodynamic scales for incorporation of D-amino acids into α R helices. Molecular mechanisms used in proteins to stabilize left-handed elements by L-amino acids are structurally enantiomeric to potential synthetic strategies for stabilizing right-handed elements with D-amino acids. However, protein elements containing short left-handed turns and helices turn out to contain useful information. D-amino acids rarely occur naturally, making it difficult to infer general rules for how they would be tolerated in proteins through an analysis of existing protein structures. The Protein Data Bank has been a reliable, rich source of information on molecular interactions and their role in protein stability and structure. Incorporating variable amino acid stereochemistry in molecular design has the potential to improve existing protein stability and create new topologies inaccessible to homochiral molecules. |
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